Research Methods : Microscopy

Brain sections processed in a variety of ways are examined and analyzed using one of two microscopes in the Smale/Nunez labs, equipped with cameras that send images to computers equipped with image analysis software. In addition, students have used a confocal microscope in a nearby common facility.

tractrace_bda_confocalFluorescent labeling for the tracer CTb (green) and the peptide orexin (red). The CTb injection was centered on the intergeniculate leaflet in the thalamus.

 

 

 

coloradommmvipplusgnrhFluorescent labeling for the tracer CTb (green) and the peptide orexin (red). The CTb injection was centered on the intergeniculate leaflet in the thalamus.

 

 

 

tract_tracing_schwartzIllustration of distribution of labeled fibers in the brain of a grass rat produced with imaging system in the Nunez lab.