Research Methods : In Situ Hybridization

In situ hybridization, is a method of localizing and detecting specific mRNA in morphologically preserved tissues sections or cell preparations by hybridizing the complementary strand of a nucleotide probe to the sequence of interest.

We use non-radioisotope cRNA probes that can give cellular level resolution.Representative images of sections labeled by in situ hybridization are shown below.

a, spatiotemporal expression pattern of clock gene Per1 in rat SCN

in-situ-per1

b, double-label of Per1 mRNA (in situ, purple) and DBP (ICC, brown) in mouse SCN

in-situ-icc-double-label