Research Methods : Immunohistochemistry

To examine the neural substrates underlying behaviors, antibodies raised against specific proteins and peptides are used to label tissue with a chromagen, typically resulting in a brown or black stain where the target protein is present.

avp_scn

A coronal view of tissue from a grass rat brain that has been stained using immunohistochemical procedures to reveal cells and fibers containing the peptide vasopressin.

nixon_oxf

A high magnification photograph of cells that have been double labeled for the detection of a peptide called orexin and a nuclear protein called c-Fos. Changes in the presence of c-Fos indicates a change in gene transcription, for example from day to night, which may differ form species to species.

lgn-1

A high magnification photograph of cells that have been double labeled for the detection of a peptide called orexin and a nuclear protein called c-Fos. Changes in the presence of c-Fos indicates a change in gene transcription, for example from day to night, which may differ frm species to species.